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03668nas a22002897a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20210420162121.0 |
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| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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ta |
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171219t2013 sp ||||| |||| 00| 0 spa | |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1888-6116 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
Salus Infirmorum |
| 245 00 - TITLE STATEMENT |
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| Title |
Estudio piloto para la reconstrucción del ligamento cruzado anterior (LCA) con cultivos de fibroblastos autólogos sobre membranas de colágeno I/III = |
| Remainder of title |
Pilot study on the anterior cruciate ligament (ACL) reconstruction with autologous fibroblasts culture on collagen I/III membranes / |
| Statement of responsibility, etc. |
López-Alcorocho Sánchez JM, Guillén García P, Rodríguez Íñigo E, Guillén Vicente I, Caballero Santos R, Guillén Vicente M, García Gómez F, Cuevas Sánchez P |
| 500 ## - GENERAL NOTE |
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| General note |
Este artículo se encuentra disponible en su edición impresa. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliografía: p.38 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Objetivo: Utilizar cultivos de fibroblastos sobre membranas de colágeno I/III para tratar la rotura del ligamento<br/>cruzado anterior (LCA).<br/>Pacientes y métodos: Estudio prospectivo con 100 biopsias de LCA de pacientes con rotura de LCA con las que<br/>se realizaron cultivos primarios de fibroblastos. Tras alcanzar 20-30 millones, se transfirieron a membranas de<br/>colágeno I/III, donde se determinó su disposición mediante tinción con hematoxilina-eosina y la expresión de los<br/>genes colágeno de tipo I (Col-I), colágeno de tipo III (Col-III), tenascina-C y Sox-9 mediante PCR en tiempo real.<br/>Resultados: A los 30 días de cultivo existía una correlación negativa entre la edad de los pacientes y la velocidad<br/>de crecimiento de los fibroblastos (p=0,003). La histología reveló que las células se disponían entre la malla formada por las fibras de las membranas, expresando Col-I, Col-III, Sox-9 y tenascina-C, características del ligamento.<br/>Conclusión: Es posible el establecimiento de cultivos primarios de fibroblastos derivados de LCA tanto in vitro<br/>como sobre membranas de colágeno I/III sin que pierdan sus características primarias de células de ligamento. <br/> |
| 520 8# - SUMMARY, ETC. |
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| Summary, etc. |
Objective: To study the possibility on the use of the in vitro cultured on I/III collagen membranes for the treatment of<br/>the broken anterior cruciate ligament (ACL).<br/>Patients and methodology: Prospective study performed with 100 ACL biopsies from patients with broken ACL, in<br/>which primary cultures of fibroblasts were established. When a number of 20-30 million was reached, they were transferred to I/III collagen membranes, where the architecture of the cells was examined by hematoxilin-eosin stained and<br/>the expression of the collagen type I (Col-I), collagen type III (Col-III), tenascin-C and Sox-9 genes by real time PCR.<br/>Results: A negative correlation between the age of the patients and the growth rate of the fibroblasts after 30 days of<br/>setting-up the culture (p=0.003) was observed. The histology revealed that the cells were disposed within the membrane fibers net, where the cells expressed the characteristic proteins of the ligament: Col-I, Col-III, Sox-9 and tenascin-C.<br/>Conclusion: It is possible to establish the primary culture of the ACL-derived fibroblasts, both in vitro and onto collagen I/III porcine membranes without losing their primary features of the ligament cells. |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
ligamento cruzado anterior |
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| Uncontrolled term |
fibroblastos |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
tenocitos |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
anterior cruciate ligament |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
fibroblasts |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
tenocytes |
| 773 ## - HOST ITEM ENTRY |
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| Related parts |
-- 2013, v. 24, n. 1, p. 33-38 |
| Title |
Trauma |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="http://www.mapfre.com/fundacion/html/revistas/trauma/v24n1/docs/Articulo5.pdf">http://www.mapfre.com/fundacion/html/revistas/trauma/v24n1/docs/Articulo5.pdf</a> |
| Link text |
Acceso al documento |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Universal Decimal Classification |
| Koha item type |
Artículo de revista |
