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20210414160451.0 |
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| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1888-6116 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
Salus Infirmorum |
| 245 00 - TITLE STATEMENT |
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| Title |
Caracterización molecular de células progenitoras del parénquima subcortical cerebral humano adulto = |
| Remainder of title |
Molecular characterization of progenitor cells from subcortical parenchyma of adult human brain / |
| Statement of responsibility, etc. |
Oliver-De La Cruz J, Carrión-Navarro J, Romaguera-Ros M, Gutiérrez-Martín A, Escobedo-Lucea C, Ayuso-Sacido A, García-Verdugo JM |
| 500 ## - GENERAL NOTE |
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| General note |
Este artículo se encuentra disponible en su edición impresa. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Bibliografía: p.128-129 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Objetivo: Caracterizar los patrones de expresión de marcadores de indiferenciación y diferenciación y las alteraciones morfológicas de las células progenitoras procedentes de parénquima cerebral humano adulto a lo largo de los pases en cultivo, evaluando su potencial para ser empleadas como fuente de progenitores de oligodendrocitos.<br/>Materiales y Métodos: Las células progenitoras se aislaron a partir de dos muestras obtenidas de pacientes sometidos a exéresis temporal por epilepsia. Para comprobar la evolución de los niveles de expresión de marcadores moleculares de diferenciación e indiferenciación en dichas células, se procedió a la extracción de ARNm en cada pase y a su estudio mediante RT-PCR. Se llevó a cabo un análisis de su capacidad proliferativa mediante inmunocitoquímica y un estudio de la evolución morfológica mediante microscopía.<br/>Resultados: Las células mostraron capacidad proliferativa durante los primeros pases en cultivo. Además, detectamos la expresión de marcadores de indiferenciación y diferenciación temprana a oligodendrocitos.<br/>Conclusión: Las células progenitoras aisladas de parénquima subcortical de cerebro humano pueden ser susceptibles de diferenciación a oligodendrocitos maduros, aunque en protocolos de diferenciación sólo deberían utilizarse pases tempranos. |
| 520 8# - SUMMARY, ETC. |
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| Summary, etc. |
Objetive: characterize the behavior of progenitor cells isolated from subcortical parenchyma of human brain in culture. We have analyzed the changes in expression patterns of differentiation/undifferentiation markers as well as cell morphology along the passages and evaluated the potential to be further used as oligodendrocyte progenitors source.<br/>Material and Methods: We isolated progenitor cells from two different samples of subcortical parenchyma human brain of patients suffering from epilepsy. Cells were kept in culture until they became quiescent/senescent.<br/>Every other passages RNAs were isolated and checked for the expression of differentiation and undifferentiation markers by using RT-PCR. Proliferation was also addressed by RT-PCR and immunocytochemistry. We carried out cell morphology studies on semithin and ultrathin sections of cells.<br/>Results: We observed decreasing proliferative capacity of both two cell lines which became quiescent/senescent around passages 8-10. We detected the expression of either undifferentiation or early neural and oligodendrocytes differentiation markers.<br/>Conclusions: As for the expression of molecular markers, progenitor cells isolated from subcortical parenchyma of human brain have the potential to differentiate into mature oligodendrocytes. |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
oligodendrocitos |
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| Uncontrolled term |
regeneración |
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| Uncontrolled term |
cerebro |
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| Uncontrolled term |
epilepsia |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
oligodendrocyte |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
regeneration |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
brain |
| 653 14 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
epilepsia |
| 773 ## - HOST ITEM ENTRY |
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| Related parts |
-- 2011, v. 22, 2, p. 122-129 |
| Title |
Trauma |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="http://www.mapfre.com/fundacion/html/revistas/trauma/v22n2/pdf/02_08.pdf">http://www.mapfre.com/fundacion/html/revistas/trauma/v22n2/pdf/02_08.pdf</a> |
| Link text |
Acceso al documento |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Universal Decimal Classification |
| Koha item type |
Artículo de revista |
